9780521836777 frontmatter

 Documents

 104 views
of 30

Please download to get full document.

View again

All materials on our website are shared by users. If you have any questions about copyright issues, please report us to resolve them. We are always happy to assist you.
Description
9780521836777 frontmatter
Share
Tags
Transcript
  RNA INTERFERENCE TECHNOLOGY RNA Interference (RNAi) technology has rapidly become one of the keymethods used in functional genomics. RNAi is used to block the expressionof genes and create phenotypes that can potentially yield clues about thefunctionofthesegenes.Inthepostgenomicera,theelucidationofthephys-iological function of genes has become the rate-limiting step in the questto develop “gene-based drugs” and RNAi could potentially play a pivotalrole in the validation of such novel drugs. In this cutting-edge overview,the basic concepts of RNAi biology are discussed, as well as the current andpotential applications. Leading experts from both academia and industryhave contributed to this invaluable reference for graduate students, post-docs, and researchers from academia wanting to initiate RNAi research intheir own labs, as well as for those working in research and developmentin biotech and pharmaceutical companies who need to understand thisemerging technology.Krishnarao Appasani is the Founder and Chief Executive Officer of Gene-Expression Systems, a gene discovery company focusing on functional ge-nomics in cancer research. © Cambridge University Presswww.cambridge.orgCambridge University Press0521836778 - RNA Interference Technology: From Basic Science to Drug DevelopmentEdited by Krishnarao AppasaniFrontmatterMore information  RNA Interference Technology FROM BASIC SCIENCE TO DRUG DEVELOPMENT Edited by KrishnaraoAppasani GeneExpression Systems, Inc. Forewords by Andrew Fire Stanford University, co-discoverer of RNAiand Marshall Nirenberg  National Institutes of HealthWinner of the Nobel Prize in Physiology or Medicine, 1968 © Cambridge University Presswww.cambridge.orgCambridge University Press0521836778 - RNA Interference Technology: From Basic Science to Drug DevelopmentEdited by Krishnarao AppasaniFrontmatterMore information  PUBLISHED BY THE PRESS SYNDICATE OF THE UNIVERSITY OF CAMBRIDGE The Pitt Building, Trumpington Street, Cambridge, United Kingdom CAMBRIDGE UNIVERSITY PRESS The Edinburgh Building, Cambridge CB2 2RU, UK40 West 20th Street, New York, NY 10011-4211, USA477 Williamstown Road, Port Melbourne, VIC 3207, AustraliaRuiz de Alarc´on 13, 28014 Madrid, SpainDock House, The Waterfront, Cape Town 8001, South Africahttp://www.cambridge.org C   Cambridge University Press 2005This book is in copyright. Subject to statutory exceptionand to the provisions of relevant collective licensing agreements,no reproduction of any part may take place withoutthe written permission of Cambridge University Press.First published 2005Printed in the United States of America Typefaces  ITC Stone Serif 9/13.5 pt. and Poppl-Laudatio  System  L A TEX2 ε  [TB]  A catalogue record for this book is available from the British Library. Library of Congress Cataloguing in Publication Data RNA interference technology : from basic science to drug development / edited byKrishnarao Appasani.p. cm.Includes bibliographical references and index.ISBN 0-521-83677-8 (alk. paper)1. Small interfering RNA. 2. Gene silencing. I. Appasani, Krishnarao.QP623.5.S63R63 2004572.8  8 – dc22 2004054562ISBN 0 521 83677 8 hardback © Cambridge University Presswww.cambridge.orgCambridge University Press0521836778 - RNA Interference Technology: From Basic Science to Drug DevelopmentEdited by Krishnarao AppasaniFrontmatterMore information   In memory of my parents For my teachers, family membersand especially my wife Shyamala and sons Raakish and Raghu © Cambridge University Presswww.cambridge.orgCambridge University Press0521836778 - RNA Interference Technology: From Basic Science to Drug DevelopmentEdited by Krishnarao AppasaniFrontmatterMore information  Contents  Foreword by   Andrew Fire  page  xi  Foreword by   Marshall Nirenberg xiii  List of Contributors  xv Introduction  1 Krishnarao Appasani Section one.  BasicRNAi,siRNA,microRNAsandgene-silencingmechanisms 1 RNAi beginnings. Overview of the pathway in C. elegans  17 Alla Grishok 2 Dicer in RNAi: Its roles  in vivo  and utility  in vitro  29  Jason W. Myers and James E. Ferrell, Jr. 3 Genes required for RNA interference  55 Nathaniel R. Dudley, Ahmad Z. Amin, and Bob Goldstein 4 MicroRNAs: A small contribution from worms  69 Amy E. Pasquinelli 5 miRNAs in the brain and the application of RNAito neurons  84 Anna M. Krichevsky, Shih-Chu Kao, Li-Huei Tsai, and Kenneth S. Kosik Section two.  Design,synthesisofsiRNAs 6 Design and synthesis of small interfering RNA (siRNA)  103 Queta Boese, William S. Marshall, and Anastasia Khvorova 7 Automated design and high throughput chemical synthesisof siRNA  118 Yerramilli V. B. K. Subrahmanyam and Eric Lader 8 Rational design of siRNAs with the Sfold software  129 Ye Ding and Charles E. Lawrence 9 Enzymatic production of small interfering RNAs  139 Muhammad Sohail and Graeme Doran vii © Cambridge University Presswww.cambridge.orgCambridge University Press0521836778 - RNA Interference Technology: From Basic Science to Drug DevelopmentEdited by Krishnarao AppasaniFrontmatterMore information
We Need Your Support
Thank you for visiting our website and your interest in our free products and services. We are nonprofit website to share and download documents. To the running of this website, we need your help to support us.

Thanks to everyone for your continued support.

No, Thanks